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a Fibrin dissolved by the fibrinolytic nanocage (FNC) was monitored by measuring the height of the remaining gel after predetermined lengths of time (0, 0.5, 1, 2, 4, 6, 8, 10, 12, or 24 h). Tris-based saline buffer (TBS) and urokinase (uPA) were used as controls. b The relative decrease in height of the fibrin gels was plotted. The results are presented as the means ± SD ( n = 3 independent experiments), and each line was obtained from the <t>exponential</t> decay equation model as described in the Methods. c To monitor nanoparticle transport across the fibrin gel upon fibrinolysis, a Transwell assay was performed. FNCs and wild-type ferritin nanocages (wFTHs) were placed on the upper chamber of the Transwell plate. The bottom chamber contained only FNC in TBS. d The wFTH transported to the bottom chamber was analyzed by western blot. e The relative intensity of the transported wFTH to the applied wFTH was plotted.
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a Fibrin dissolved by the fibrinolytic nanocage (FNC) was monitored by measuring the height of the remaining gel after predetermined lengths of time (0, 0.5, 1, 2, 4, 6, 8, 10, 12, or 24 h). Tris-based saline buffer (TBS) and urokinase (uPA) were used as controls. b The relative decrease in height of the fibrin gels was plotted. The results are presented as the means ± SD ( n = 3 independent experiments), and each line was obtained from the exponential decay equation model as described in the Methods. c To monitor nanoparticle transport across the fibrin gel upon fibrinolysis, a Transwell assay was performed. FNCs and wild-type ferritin nanocages (wFTHs) were placed on the upper chamber of the Transwell plate. The bottom chamber contained only FNC in TBS. d The wFTH transported to the bottom chamber was analyzed by western blot. e The relative intensity of the transported wFTH to the applied wFTH was plotted.

Journal: Experimental & Molecular Medicine

Article Title: Fibrinolytic nanocages dissolve clots in the tumor microenvironment, improving the distribution and therapeutic efficacy of anticancer drugs

doi: 10.1038/s12276-021-00688-7

Figure Lengend Snippet: a Fibrin dissolved by the fibrinolytic nanocage (FNC) was monitored by measuring the height of the remaining gel after predetermined lengths of time (0, 0.5, 1, 2, 4, 6, 8, 10, 12, or 24 h). Tris-based saline buffer (TBS) and urokinase (uPA) were used as controls. b The relative decrease in height of the fibrin gels was plotted. The results are presented as the means ± SD ( n = 3 independent experiments), and each line was obtained from the exponential decay equation model as described in the Methods. c To monitor nanoparticle transport across the fibrin gel upon fibrinolysis, a Transwell assay was performed. FNCs and wild-type ferritin nanocages (wFTHs) were placed on the upper chamber of the Transwell plate. The bottom chamber contained only FNC in TBS. d The wFTH transported to the bottom chamber was analyzed by western blot. e The relative intensity of the transported wFTH to the applied wFTH was plotted.

Article Snippet: The fibrin decay of each sample was analyzed using the exponential decay equation (GraphPad v. 9.1.2).

Techniques: Saline, Transwell Assay, Western Blot

Fibrin decay parameters using the exponential decay equation.

Journal: Experimental & Molecular Medicine

Article Title: Fibrinolytic nanocages dissolve clots in the tumor microenvironment, improving the distribution and therapeutic efficacy of anticancer drugs

doi: 10.1038/s12276-021-00688-7

Figure Lengend Snippet: Fibrin decay parameters using the exponential decay equation.

Article Snippet: The fibrin decay of each sample was analyzed using the exponential decay equation (GraphPad v. 9.1.2).

Techniques: Saline